TRANSFLY: Seasonal transitions in the fruit fly pest Drosophila suzukii Objectivesidentify the behavioural, neural and morphological mechanisms underlying seasonal adaptation in Drosophila suzukiidetermine how environmental factors drive the transition between summer and winter phenotypescharacterize structural and functional plasticity in the olfactory system using advanced neuroimagingdevelop AI-based tools for automated identification of seasonal phenotypes in the fieldimprove integrated pest management through phenotype-specific monitoring and control strategiesMethodslaboratory breeding of summer and winter phenotypes under controlled environmental conditionsbehavioural assays to study host-seeking, feeding, oviposition and pheromone-guided behaviourchemical ecology using GC-MS, GC-EAD and electrophysiologyin vivo two-photon structural and functional neuroimaging of the olfactory systemAI-based phenotyping of seasonal morphs from field trap imagesfield monitoring and validation of pest management strategies in orchards and vineyardsProject coordinatorSergio Angeli (Entomology and Fruit Analysis Lab, Free University of Bozen-Bolzano)PartnersGianfranco Anfora (Center for Agricultural, Environmental and Food Sciences (C3A), University of Trento) Silvia Schmidt, Urban Spitaler (Biological Plant Protection Methods Working Group, Laimburg Research Centre)External partnersErnst Wimmer (Department of Developmental Biology, Georg-August University Göttingen, Germany)Alan Dorin (Department of Data Science & AI, Monash University, Melbourne, Australia)Financing3-year collaborative research project funded by the Autonomous Province of Bolzano QUMARE: Verification of the Quantum theory of Magnetoreception in a new animal model, a biological magnetic sensor. Objectivesquantify magnetosensitive behaviours in beesidentify the magnetosensitive neurons by functional brain imagingcompare experimental findings with the prediction of the radical pair theoryProject coordinatorAlbrecht HaasePartnersCNR, Institute of Materials for Electronics and Magnetism (IMEM), Parma/Trento, Riccardo Cabassi, Roberto VerucciQuantum Biology and Computational Physics research group, University of Oldenburg, Germany, headed by Ilia Solov'yovFinancing4 years PhD fellowship from CIMeC Doctoral School 2025 - 20293 years PhD fellowship from Q@TN - Quantum Science and Technology in Trento 2022 - 2025 The role of the microbiota on locomotion, cognitive abilities, and health of the honey bee (MicroBee) Objectivesdevelop methods to manipulate the honeybee microbiotainvestigate the dependence of behaviour on the microbiota in honeybeesstudy the gut-brain interaction with neuroimaging methodsMethodscreate germ-reduced and germ-manipulated honeybeescomparative characterization of the honeybee microbiotalocomotion and learning assays functional brain imagingProject coordinatorElisa Frasnelli (CIMeC)PartnersAlbrecht HaaseGianfranco Anfora (C3A)Omar Rota Stabelli (C3A)FinancingFondazione Cassa di Risparmio di Trento e Rovereto CARITRO (2024-2026)University of Trento Internal call 2023 IBC: Impollinatori, Biodiversità e Cambiamento climatico - Pollinators, Biodiversity and Climate Change Objectivesdevelop experimental setups allowing to perform experiments under controlled enhanced temperature conditionsinvestigate the role of thermal stress on odour perception and learning & memoryMethodsautomated learning assays at elevated temperaturesfunctional brain imaging at elevated temperturesProject coordinatorsAlbrecht Haase and Elisa Frasnelli (CIMeC)FinancingFondazione Cassa Rurale di Trento (2024-2027)5xMILLE University of Trento 2023 Functional brain imaging in a novel transgenic model of Drosophila suzukii Objectivesestablish two-photon brain imaging methods in D. suzukii.olfactory systemidentify common features in the neuronal code of attractive and aversive odoursapply those in future pest control strategies against D. suzukii. Project coordinatorsAlbrecht HaasePartnersResearch unit for Entomology and agricultural zoology, C3A - Center Agriculture Food Environment, University of Trento, headed by Gianfranco AnforaResearch lab for Evolution and Development of Morphology and Behaviour, Institute of Developmental Biology of Marseille (IBDM), CNRS, France, headed by Benjamin Prud’hommeFinancing2 years Post-doc fellowship from Fondazione Caritro 2021 - 2023 Genetically engineering of biomarkers in the bee brain ObjectivesDevelopment of a completely new experimental platform providing genetically engineered brain-markers for non-invasive stainingFast 3D imaging of odour-induced activities in specific neurones selected by gene promotorsRecording calcium activity patterns simultaneously in multiple neuropiles like the antennal lobe, the mushroom body (MB) and the lateral horn (LH).PartnersUniversity of Düsseldorf, Institute for Evolutionary Genetics, Germany, Research group for Evolutionary Genetics headed by Martin BeyeCentre National de 'la Recherche Scientifique (CNRS), France, Evolbee Research Team (Evolution; Genomes, Behavior and Ecology) headed by Jean-Christophe SandozFinancing2 years Post-doc fellowship financed by CIMeC 2021 - 2023 PRIN Partner manipulation in plant-insect relationships ObjectivesHow do plant-derived chemicals affect animal behaviour?Have neuroactive compounds produced by plants evolved not (or not only) as a mere deterrent for animals, but also as a plant tool to attract and manipulate animal behaviour?Plant-animal interaction from new ecological and neurobiological perspectivesModel: ant-plant relationships mediated by extra-floral nectar (EFN)Combined approach involving plant chemistry and physiology, ethology and neurobiology, transcriptomic and microbial ecologyPartnersInternational Laboratory of Plant Neurobiology, University of Firenze, Stefano Mancuso (Coordinator)Department of Agricultural and Environmental Sciences - Production, Landscape, Agroenergy, Università degli Studi di Milano, Franco FaoroMyrmecology Lab, Department of Chemistry, Life Sciences and Environmental Sustainability, Università degli Studi di Parma, Donato Antonio GrassoDepartment of Life Sciences, Università degli Studi di Siena, Massimo NepiFinancing3 years project financed by National PRIN program of the Ministry of Education, Universities and Research 2019 - 2022 BRANDY: Brain Network Dynamics BRANDY Project pageMethodscoordination of common teaching activitiessupport of students’ interest and engagement in brain network dynamics throughout all stages of their early academic carrierformation of a collaborative environment for young academicsObjectivesFoundation of a Strategic Unit for the Interdisciplinary Research on Brain Network DynamicsCreation of a cluster of experts in this fast-growing field of researchDevelopment of an infrastructure, optimized for information exchange among the partnersFormation of a collaborative environment for young academics with different skills for common research initiativesGeneration of visibility of our actions for the international research communityProject coordinatorAlbrecht Haase (DF & CIMeC)PartnersCenter for Mind/Brain Sciences (CIMeC)Unit leader: Jorge JovicichProject members: Daniel Baldauf, Ludovico Minati, Carlo MiniussiDepartment of Physics (DF)Unit leader: Paolo BettottiProject members: Renzo Antolini, Lorenzo Pavesi, Leonardo RicciDepartment of Information Engineering and Computer Sciences (DISI)Unit Leader: Lorenzo BruzzoneProject member: Nicu SebeCentre for Integrative Biology (CIBIO)Unit Leader: Marco CanossaFondazione Bruno Kessler (FBK)Paolo Avesani, Luca Faes, Emanuele OlivettiItalian Institute of Technology (IIT)Angelo Bifone, Alessandro Gozzi, Stefano PanzeriFinancing3 year strategic project financed by the Strategic Plan 2017-2021 of the University of Trento STEFANIE: Sublethal effects of neonicotinoids on the honeybee brain: from single neuron imaging to colony studies STEFANIE Project pageMethodsfunctional imaging of different regions along the odour processing pathwaycontrolled perfusion of the brain with active substances during imagingcomparative behavioural studies using PERObjectivesdetermine brain regions sensitive to neonicotinoid pesticidesquantify relevant doses for the onset of effects in the olfactory systemcompare effects in the brain to behavioural symptoms in field studiesProject coordinatorAlbrecht Haase (DF & CIMeC)PartnersSergio Angeli (University of Bolzano)Gianfranco Anfora (FEM)Paolo Fontana and Valeria Malagnini (FEM)Financing3 year project financed by the Provincia Autonoma di Bolzano Of bees and men: Development of an optogenetic animal model to study oscillatory neural networks Methodshigh temporal resolution imaging of responses to odour stimulianalysis of oscillatory activity in the honeybee braindevelopment of an optogenetic toolbox for real-time modulation of the olfactory processing networkObjectivestemporal coding in odour perceptionconnectivity in the olfactory networkchanges in the network properties under optogenetic modulation of single nodesProject coordinatorAlbrecht Haase (DF & CIMeC)PartnersNathan Weisz (University of Salzburg)Paolo Macchi and Anna Cereseto (CIBIO)Renzo Antolini (DF)Luca Faes (FBK) and Giandomenico Nollo (DII)Gianfranco Anfora (FEM)Financingas a Strategic Project by the Università degli Studi di Trento Fundamental processes in odorant receptor interaction: beyond the shape theory of olfaction Methodsstudying odour coding of compounds that share chemical properties but differ in molecular vibration and vice versadevelope in vitro assays to study odour receptor interactionObjectivestrace back insects' ability to distinguish chemically identical odour compoundsstudy possible influence of molecular vibrationsexclude alternative explanations for differential odour codingPartnersPaolo Macchi (CIBIO)Renzo Antolini (DF)Luca Turin and Efthimios Skoulakis (BSRC "Alexander Fleming", Vari, Greece)Marco Paoli and Giovanni Galizia (University of Konstanz)Financingsupported by an EMBO Short-Term Fellowship ASTF 108-2015 IBRAIM: Insect brain imaging from fundamental neuroscience to pest control Methodstwo-photon in vivo imaging of insect brains in vivo to outperform conventional microscopy by spatial and temporal resolution, penetration depth and photodamage limitationObjectivesOdour coding principles in the honeybee brainMorphological and functional plasticity in the honeybee brain after associative or non-associative learningIn of odour perception due to neurotoxic pesticides as a possible cause for the western world honeybee population decline (Colony Collapse Disorder)Project coordinatorAlbrecht Haase (DF & CIMeC)Financing as Research unit (IBRAIM) by the Provincia Autonoma di Trento Sustainable strategies against infesting flies: from olfactory perception to a safeguard of small fruits in Trentino Methodsbehaviour experiments in Drosophila melanogaster and Drosophila suzukiioptical imaging of transgenetic fruitfliesObjectivesdifferences between D. suzukii e D. melanogaster regarding reception, behaviour, and neuroanatomy.development of sustainable strategies to explore these results to counteract the diffusion of D. suzukii.Project coordinatorAlbrecht Haase (DF & CIMeC)PartnersElisabetta Versace and Giorgio Vallortigara (ACN lab, CIMeC)Gianfranco Anfora (FEM)Mauro Mandrioli (University of Brescia)Financingby the Communal Government of the City of Rovereto Multifunctional nanohybrid photosensitizers for Photodynamic therapy via X-ray and IR activation Methodssynthesis and functionalization of photo-sensitive silicon Nanowiresin vitro experiments on IR activation, and quantification of phototoxic effectsObjectivesdevelopment of a photosensitizer that is flexibly activatable at very different wavelengthcharacterization of functionality in cell culturesProject coordinatorRoberto Verucchi (Institute of materials for electronics and magnetism, CNR)PartnersRoberta Tatti (IMEM, CNR)Albrecht Haase and Renzo Antolini (DF and CIMeC)Thomas Paternoster and Nicola Berti (Sandoz Industries)Financing by Fondazione Cassa di Risparmio di Trento e Rovereto